Author(s):
1. Marko Koprena, Tehnološki fakultet Univerziteta u Banjoj Luci, Republic of Srpska, Bosnia and Herzegovina
2. Vladimir Mlinarević, Tehnološki fakultet Univerziteta u Banjoj Luci, Republic of Srpska, Bosnia and Herzegovina

Abstract:
Vezivanjem ljekovitih preparata na polimerne nosače dobijaju se biološkia aktivni materijali. Njihove osobine, kinetika vezivanja i hemizam zavise od vrste nosača i od strukture samog lijeka. U ovom radu selektivnom oksidacijom celuloznog zavoja dobijena je oksidovana celuloza (OC) sa 9,896% COOH. Oksidacija je vršena smjesom HNO3/H3PO4 2:1 i 1,43 % NaNO2 u trajanju od 20h na temperaturi od 26°C Ovako dobijena OC iskorištena je za hemijsko vezivanje anelgetika diklofenaka u obliku kalijumove soli. Vezivanje lijeka vršeno je iz vodenog rastvora anelgetika koncentracija c=1,5∙10-3, 3,0∙10-3 i 4,5∙10-3 mol/L na temperaturi 26 °C, a desorpcija lijeka u fiziološkom rastvoru. Količine vezanog i otpuštenog lijeka određene su spektrofotometrijski u UV području koristeći UV-VIS spectrofotometar Perkin Elmer model Lambda 25, na talasnoj dužini λmax 276 nm. Maksimalna količina vezanog lijeka (229,442 mg/g OC) dobijena je sorpcijom na oksidovani zavoj iz rasvora c=4,5∙10-3, a maksimalna količina desorbovanog diklofenaka iznosila je 46,114 mg/g. U radu se proučava uticaj hemijske strukture diklofenaka, pH vrijednosti rastvora iz koga se vrši sorpcija kao i trajanja sorpcije na količinu vezanog lijeka. Ustanovljeno je da se vezivanje lijeka ostvaruje jonskim i vodoničnim vezama lijeka sa oksidovanim celuloznim zavojem. Takođe, količina vezanog diklofenaka pokušala se povećati vezivanjem Na+ na oksidovanu celulozu, ali je zbog prevelikog sadržaja karboksilnih grupa došlo do rastvaranja OC. Making a bond of a medicinal preparation to the polymer supports results in getting bioactive materials. Their properties, kinetics and chemism depend on support type and drug's structure. In this paper, an oxidized cellulose (OC) with 9,896% COOH is produced by using selective oxidation of a cellulose-based bandage. Oxidation has been carried out in mixture of HNO3/H3PO4 2:1 and 1,43% NaNO2 for 20h on 26°C. This oxidized cellulose has been used for chemical bonding of an analgesic diclofenac, a derivative of potassium salt. The chemical bonding of the drug has been done from an analgesic solution with concentrations c=1,5∙10-3, 3,0∙10-3 i 4,5∙10-3 mol/L, on temperature of 26±1 ˚C while desorption was performed in physiological solution. The amounts of bonded and released antibiotic were determined spectrophotometrically in UV range by using UV-VIS spectrophotometer Perkin Elmer model Lambda 25, with a wavelength of λmax 276 nm. The maximum amount of bonded drug (229.442 mg/g OC) has been obtained by a sorption from the solution of concentration c=4,5∙10-3 to the oxidized bandage, while the greatest amount of desorbed diclofenac was 46.114 mg/g. The paper studies the influence of diclofenac chemical structure, pH of solution in which sorption is performed and sorption duration, on the amount of bonded drug. It was established that the drug bonding was achieved by ionic bonds and the hydrogen bonds of the drug functional groups with oxidised cellulose bandage. Furthermore, the amount of bonded diclofenac was tried to rise by attachment of Na+ to OC, however it has been come to dissolution of OC due to high content of the carboxylic (-COOH) groups.

Key words:
Oksidovana celuloza, diklofenak-K, hemizam vezivanja, sorpcija katjona,Oxidized cellulose, diclofenac-K, chemism of bonding, sorption of cations.

Thematic field:
Medical and health sciences

Date of abstract submission:
06.10.2017.

Conference:
Studenti u susret nauci 2017